Dominic Winter
Dr. Dominic Winter
  • Institut für Biochemie und Molekularbiologie
Research topics
  • lysosomes
  • proteomics
  • biochemistry
We are investigating the lysosome, the central lytic organelle of mammalian cells, with mass spectrometry-based proteomics and biochemistry/molecular biology approaches. For this purpose, we are developing novel mass spectrometry strategies for the enrichment of lysosomes and the characterization of lysosomal proteins. These methods include the relative and absolute quantification of proteins and posttranslational modifications, the identification of novel lysosomal proteins, the investigation of cell type- and organelle-specific proteins, the identification of protein-protein interactions, and the structural characterization of lysosomal proteins and protein complexes. We apply these techniques to the investigation of cell- and organ-specific differences in lysosomal protein composition, transcriptional regulation of lysosomal proteins, and the effect of pathological conditions on lysosomal composition and signaling.
Selected publications

Akter F, Bonini S, Ponnaiyan S, Kögler-Mohrbacher B, Bleibaum F, Damme M, Renard BY and Winter D, Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteins. Molecular & Cellular Proteomics 2023, 22, 100509.

Singh J, Elhabashy H, Muthukottiappan P, Stepath M, Eisenacher M, Kohlbacher O, Gieselmann V and Winter D, Cross-linking of the Endolysosomal System Reveals Flotillin Structures and Potential Cargo. Nature Communications 2022, 13, 6212.

Richards CM, Jabs S, Qiao W, Varanese LD, Schweizer M, Mosen PR, Riley NM, Zengel JR, Flynn RA, Rustagi A, Widen JC, Peters CE, Ooi YS, Xie X, Shi PY, Bartenschlager R, Bogyo M, Bertozzi CR, Blish CA, Winter D, Nagamine CM, Braulke T and Carette J, The human disease gene LYSET is essential for lysosomal transport and viral infection. Science 2022, 378, eabn5648.

Ponnaiyan S, Akter F, Singh J and Winter D, Comprehensive draft of the mouse embryonic fibroblast lysosomal proteome by mass spectrometry based proteomics. Scientific Data 2020, 7 (1), 68.

Dominic Winter
Dr. Dominic Winter
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